Jennifer Barnhart is a veteran puppeteer who took over the role of Gladys the Cow on Sesame Street. Barnhart, who received her BFA in puppetry from the University of Connecticut, began her puppetry career working on the Animal Planet series "Once Upon a Tree" and subsequently played Cleo the Lion on Between the Lions, before joining the cast of Sesame Street. Barnhart was the only member of the original cast performing on the Broadway puppet musical Avenue Q, continuing her original roles of Mrs. Thistletwat and all-purpose assistant, and understudying the roles of Kate Monster and Lucy T. Slut, until the show closed in 2009 .
Barnhart also performed on John Tartaglia's Johnny and the Sprites.
- Elmo's World: Wild Wild West!
- Elmo's Christmas Countdown
- Late Night with Jimmy Fallon
- season 5
- Dr. Teeth (puppetry only) 2014
- Muppets on Broadway (2017)